Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (SPM530).
Flow Cytometry of Jurkat cells using Bcl-2 Monoclonal Antibody (SPM530) (red) and isotype control (green).
Western Blot analysis of Bcl-2 in human skin using Bcl-2 Monoclonal Antibody (SPM530).
Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Mouse Monoclonal Antibody (SPM530).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.