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Home >> Antibodies >> Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Mouse Monoclonal Antibody [Clone 124]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 Mouse Monoclonal Antibody (124).

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Intracellular staining of Bcl-2 in Jurkat cells Bcl-2 Mouse Monoclonal Antibody (124) (red) and isotype control (green).

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Western Blot Analysis of Bcl-2 in human skin Bcl-2 Mouse Monoclonal Antibody (124).

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SDS-PAGE Analysis Purified Bcl-2 Mouse Monoclonal Antibody (124). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of MCF-7 cells using Bcl-2 Mouse Monoclonal Antibody (124).

Product Details

Synonyms

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Positive Control

Jurkat, K562, HL-60 or HeLa cells. Tonsil or follicular lymphomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein

Cellular Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25-26kDa

Specificity & Comments

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pezzella F et. al. American Journal of Pathology, 1990, 137(2):225-32.

Bioinformatics

  •  Entrez Gene ID: 596
  •  Gene Symbol: BCL2
  •  SwissProt: P10415
  •  Chromosome Location: 18q21.33
  •  Unigene: 150749
NBT00530