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Home >> Antibodies >> AIF1 / Iba1 (Microglia Marker)

AIF1 / Iba1 (Microglia Marker)

Mouse Monoclonal Antibody [Clone AIF1/1909]

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Catalog No Formulation Size Price (USD)  
199-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
199-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
199-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 199
Human SwissProt P55008
Human Unigene 76364
Human Gene Symbol AIF1
Human Chromosome Location 6p21.33
Synonyms Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
Immunogen Purified fragment of human recombinant AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 17kDa
Cellular Localization Cytoplasmic and Cell Surface
Species Reactivity Human. Others not known.

Specificity & Comments

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Lymph Node, Tonsil or Kidney

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Kelemen SE, Autieri MV. Expression of allograft inflammatory factor-1 in T lymphocytes. Am J Pathol2005; 167: 619-26.
DOC#01909