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Home >> Antibodies >> ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker)

ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker)

Recombinant Mouse Monoclonal Antibody [Clone r57]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Pituitary Gland stained with ACTH Mouse Recombinant Monoclonal Antibody (r57).

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SDS-PAGE Analysis Purified ACTH Mouse Recombinant Monoclonal Antibody (r57). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Adrenocorticotropin; alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin; beta-Endorphin; beta or gamma Lipotropin (LPH); CLIP; Met Enkephalin; POC; POMC

Positive Control

Normal pituitary gland or pituitary tumor.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

N-terminal fragment of human ACTH conjugated to KLH

Cellular Localization

Cytoplasm and Secreted

Species Reactivity

Human. Mouse. Rat. Expected to show a broad species reactivity.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Specificity & Comments

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. '

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200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hsu DW et. al. American Journal of Pathology, 1991, 138(4):897-909.

Bioinformatics

  •  Entrez Gene ID: 5443
  •  Gene Symbol: POMC
  •  SwissProt: P01189
  •  Chromosome Location: 2p23.3
  •  Unigene: 1897
NBT01380