Formalin-fixed, paraffin-embedded human lymphoma stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/3921R).

Formalin-fixed, paraffin-embedded human lymphoma stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/3921R).

Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/3921R).

ZAP70 (Chronic Lymphocytic Leukemia Marker) [ZAP70/3921R]

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Summary

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
ZAP70
Mol. Weight of Antigen
70kDa
Immunogen
Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID
7535
Unigene
234569
SwissProt
P43403

Additional Information

Clone
ZAP70/3921R
Human Gene Symbol
ZAP70
Chromosome Location
2q12

Known Applications & Suggested Dilutions

  • ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Iwashima M, et al. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 1994;263:1136-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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