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Recombinant TLE1 (Synovial Sarcoma Marker) [TLE1/8007R]

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Summary

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein �1 (TLE1) is a protein that is �encoded by the �TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG, kappa
Cellular Localization
Nucleus.
Gene Name
TLE1
Positive Control
HeLa|HepG2 or Jurkat cells. Human endometrium carcinoma or sarcomas.
Immunogen
Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
Alternate Names
Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1

Database Links

Entrez Gene ID
7088
Unigene
197320; 689805
SwissProt
Q04724

Additional Information

Clone
TLE1/8007R
Human Gene Symbol
TLE1
Chromosome Location
9q21.32
Mol. Weight of Antigen
83kDa

Functions

  • Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Jagdis A, et al. Am J Surg Pathol. 2009; 33:1743-51.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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