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Recombinant TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody [TIMP2/2488R]

In Stock
Catalog Number Formulation Size Price
7077-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7077-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7077-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
Positive Control
Colon, Endometrial, HeLa cells. Normal Placenta. Breast, Prostate or Ovarian Carcinoma.
Immunogen
Synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)
Alternate Names
Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinase 2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
TIMP2/2488R
Chromosome Location
17q25
Mol. Weight of Antigen
21kDa

Functions

  • Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Key References

  • Murray et al. 1998. Gut. 43(6):791-7.
  • Jeffery et al. 2009. Histopathology. 54(7):820-8

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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