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TGFB2 (Transforming Growth Factor beta 2) Antibody [TGFB2/1679]

In Stock
Catalog Number Formulation Size Price
7042-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7042-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7042-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
TGFB2 (Transforming Growth Factor beta 2) Antibody

SDS-PAGE Analysis of Purified TGF beta 2 Mouse Monoclonal Antibody (TGFB2/1679). Confirmation of Purity and Integrity of Antibody.

TGFB2 (Transforming Growth Factor beta 2) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with TGF beta 2 Mouse Monoclonal Antibody (TGFB2/1679). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Transforming growth factor bs (TGFbs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFa. It is now realized that TGFbs mediate many cell-cell interactions that occur during embryonic development. Three TGFbs have been identified in mammals. TGFb1, TGFb2 and TGFb3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFb requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFb3 protein has approximately 80% identity to the mature region of both TGFb1 and TGFb2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, AKT Signaling, Angiogenesis, Colon Cancer, Complement System, MAPK Signaling, Mesenchymal Stem Cell Differentiation, Neuroinflammation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted.
Gene Name
TGFB3
Positive Control
Prostate or lung carcinoma.
Immunogen
Recombinant full-length human TGFB2 protein
Alternate Names
Transforming growth factor beta-3 proprotein | Latency-associated peptide | Transforming growth factor beta-3, Transforming Growth Factor beta 2; TGF b2

Database Links

Entrez Gene ID
7042
SwissProt
P10600

Additional Information

Clone
TGFB2/1679
Chromosome Location
1q41
Mol. Weight of Antigen
13kDa

Related Antibodies

View All TGFB3 Antibodies

Functions

  • Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.
  • Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).
  • Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).

Key References

  • Miller, D.A., et al. 1990. Ann. N.Y. Acad. Sci. 593: 208-217.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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