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Recombinant p53 (Tumor Suppressor Protein) Antibody [rIMX25]

In Stock
Catalog Number Formulation Size Price
7157-MSM37-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
7157-MSM37-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
7157-MSM37-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
p53 Antibody in Immunohistochemistry (IHC (P))

p53 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with p53 Recombinant Mouse Monoclonal Antibody (rIMX25). Inset: PBS instead of primary antibody; secondary only negative control.

p53 Antibody in SDS-PAGE

p53 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified p53 Recombinant Mouse Monoclonal Antibody (rIMX25). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The accumulation of p53 protein in response to DNA damage in vitro is well established and appears to induce growth arrest and apoptosis by the transcriptional regulation of other genes. In irradiated mice, p53 protein accumulates in splenocytes, thymocytes and osteocytes, though not in hepatocytes. Mouse T3T3 cells express high levels of phenotypically characteristic wild type p53 protein which carries two missense mutations. The range of antigenic sites in mouse p53 protein and human p53 protein is very similar.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human, Mouse
Research Areas
Breast Cancer, Cardiovascular, Immunology, AKT Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Defective Intrinsic Apoptosis, Infectious Disease, Lung Cancer, MAPK Signaling, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
TP53
Positive Control
Human prostate or colon. Mouse T3T3 cells.
Immunogen
Prokaryotic recombinant protein corresponding to the full length wild type mouse p53 protein
Alternate Names
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53)

Database Links

Entrez Gene ID
7157
SwissProt
P04637

Additional Information

Clone
rIMX25
Chromosome Location
17p13.1
Mol. Weight of Antigen
53kDa

Related Antibodies

View All TP53 Antibodies

Functions

  • Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652). Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Key References

  • Lane D P, Stephen C W, Midgley C A, et al. Oncogene. 12: 2461–2466 (1996).
  • Midgley C A, Owens B, Briscoe C V, et al. Journal of Cell Science. 108: 1843–1848 (1995).
  • Milner J, Chan Y S, Medcalf E A, et al. Oncogene. 8: 2001–2008 (1993).
  • Midgley C A, Fisher C J, Bártek J, et al. Journal of Cell Science. 101: 183–189 (1992).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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