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Recombinant CD95 / FAS / TNFRSF6 (Marker of Cancer Associated Fibroblasts) Antibody [rGM30]

In Stock
Catalog Number Formulation Size Price
355-MSM21-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
355-MSM21-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
355-MSM21-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD95 Antibody in SDS-PAGE

CD95 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD95 Recombinant Mouse Monoclonal Antibody (rGM30). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Fas, also known as CD95 antigen, is a 48 kD transmembrane glycoprotein. It is a member of the nerve growth factor receptor/tumor necrosis factor superfamily. This cell surface molecule mediates receptor-triggered apoptosis (programmed cell death). During embryonic and postembryonic development, many cells die by means of programmed cell death, which plays a major role in determining morphological and functional maturity in a variety of systems, including the formation of the neural network and clonal deletion of autoreactive T cells. Apoptosis is accompanied by condensation of the cytoplasm, loss of plasma membrane microvilli and extensive degradation of chromosomal DNA into oligomers of about 180 base pairs. The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, AKT Signaling, Complement System, Endothelial Cell Marker, MAPK Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
FAS
Positive Control
MCF-7 cells. Human hepatocellular or bladder carcinoma.
Immunogen
Prokaryotic recombinant fusion protein corresponding to the internal domain near the C-terminal end of the human Fas molecule
Alternate Names
Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

Database Links

Entrez Gene ID
355
SwissProt
P25445

Additional Information

Clone
rGM30
Chromosome Location
10q24.1
Mol. Weight of Antigen
38-50kDa

Related Antibodies

View All FAS Antibodies

Functions

  • Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Key References

  • Sasaki et al. Br J Urol 81: 852, 1998.
  • Sugihara et al. Anticancer Res 17: 3861, 1997.
  • Sasaki, Y et al. “Immunohistochemical study of Fas, Fas ligand and interleukin-1 beta converting enzyme expression in human prostatic cancer.” British journal of urology vol. 81,6 (1998): 852-5. doi:10.1046/j.1464-410x.1998.00665.x
  • Sugihara, A et al. “Expression of Fas and Fas ligand in the testes and testicular germ cell tumors: an immunohistochemical study.” Anticancer research vol. 17,5B (1997): 3861-5.
  • Nagata, S, and P Golstein. “The Fas death factor.” Science (New York, N.Y.) vol. 267,5203 (1995): 1449-56. doi:10.1126/science.7533326
  • Itoh, N, and S Nagata. “A novel protein domain required for apoptosis. Mutational analysis of human Fas antigen.” The Journal of biological chemistry vol. 268,15 (1993): 10932-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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