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Recombinant CD83 (Marker of Activated Dendritic Cells) Antibody [r1H4b]

In Stock
Catalog Number Formulation Size Price
9308-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
9308-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
9308-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD83 Antibody in Immunohistochemistry (IHC (P))

CD83 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD83 Recombinant Mouse Monoclonal Antibody (r1H4b). Inset: PBS instead of primary antibody; secondary only negative control.

CD83 Antibody in SDS-PAGE

CD83 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD83 Recombinant Mouse Monoclonal Antibody (r1H4b). Confirmation of Purity and Integrity of Antibody.

CD83 Antibody in Western Blot (WB)

CD83 Antibody in Western Blot (WB)

Western Blot Analysis of RH-30 and PC-3 cell lysates using CD83 Recombinant Mouse Monoclonal Antibody (r1H4b).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD83 antigen, a member of the immunoglobulin superfamily, is a useful marker of mature and activated dendritic cells. These include Langerhans cells in the skin, peripheral blood dendritic cells and interdigitating reticulum cells within the T cell zones of lymphoid organs. Human early pregnancy decidua contains immunostimulatory CD83 antigen positive dendritic cells. Some germinal centre B cells and activated peripheral lymphocytes also express CD83 antigen. CD83 is also used as a marker for Hodgkin’s lymphoma, Reed Sternberg cells and breast carcinoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immuno Oncology, Immune checkpoint
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Membrane
Gene Name
CD83
Positive Control
Human brain, tonsil, lymph node or spleen. RH30 or PC-3 cell lysates.
Immunogen
Prokaryotic recombinant protein corresponding to 124 amino acids of the external N-terminus of the CD83 molecule.
Alternate Names
CD83 antigen, CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD83, BL11; HB15; B-cell activation protein

Database Links

Entrez Gene ID
9308
SwissProt
Q01151

Additional Information

Clone
r1H4b
Chromosome Location
6p23
Mol. Weight of Antigen
23kDa

Related Antibodies

View All CD83 Antibodies

Functions

  • May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Key References

  • Batool, Aalia et al. “CD83, a Novel MAPK Signaling Pathway Interactor, Determines Ovarian Cancer Cell Fate.” Cancers vol. 12,8 2269. 13 Aug. 2020, doi:10.3390/cancers12082269
  • Küppers, Ralf. “CD83 in Hodgkin lymphoma.” Haematologica vol. 103,4 (2018): 561-562. doi:10.3324/haematol.2018.188870
  • Wu, Zhiwen et al. “CD83 expression characterizes precursor exhausted T cell population.” Communications biology vol. 6,1 258. 11 Mar. 2023, doi:10.1038/s42003-023-04631-6
  • Kämmerer, U et al. “Human decidua contains potent immunostimulatory CD83(+) dendritic cells.” The American journal of pathology vol. 157,1 (2000): 159-69. doi:10.1016/S0002-9440(10)64527-0
  • Bell, D et al. “In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas.” The Journal of experimental medicine vol. 190,10 (1999): 1417-26. doi:10.1084/jem.190.10.1417
  • Takahashi, K et al. “Heterogeneity of dendritic cells in human superficial lymph node: in vitro maturation of immature dendritic cells into mature or activated interdigitating reticulum cells.” The American journal of pathology vol. 153,3 (1998): 745-55. doi:10.1016/S0002-9440(10)65618-0
  • Sorg, U R et al. “Hodgkin's cells express CD83, a dendritic cell lineage associated antigen.” Pathology vol. 29,3 (1997): 294-9. doi:10.1080/00313029700169125
  • Thurnher, M et al. “Human renal-cell carcinoma tissue contains dendritic cells.” International journal of cancer vol. 68,1 (1996): 1-7. doi:10.1002/(SICI)1097-0215(19960927)68:1<1::AID-IJC1>3.0.CO;2-V
  • Zhou, L J, and T F Tedder. “Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily.” Journal of immunology (Baltimore, Md. : 1950) vol. 154,8 (1995): 3821-35.
  • Zhou, L J et al. “A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily.” Journal of immunology (Baltimore, Md. : 1950) vol. 149,2 (1992): 735-42.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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