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Recombinant CD83 (Marker of Activated Dendritic Cells) Antibody [r1H4b]

In Stock
Catalog Number Formulation Size Price
9308-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
9308-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
9308-MSM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD83 Antibody in Immunohistochemistry (IHC (P))

CD83 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD83 Recombinant Mouse Monoclonal Antibody (r1H4b). Inset: PBS instead of primary antibody; secondary only negative control.

CD83 Antibody in SDS-PAGE

CD83 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD83 Recombinant Mouse Monoclonal Antibody (r1H4b). Confirmation of Purity and Integrity of Antibody.

CD83 Antibody in Western Blot (WB)

CD83 Antibody in Western Blot (WB)

Western Blot Analysis of RH-30 and PC-3 cell lysates using CD83 Recombinant Mouse Monoclonal Antibody (r1H4b).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD83 antigen, a member of the immunoglobulin superfamily, is a useful marker of mature and activated dendritic cells. These include Langerhans cells in the skin, peripheral blood dendritic cells and interdigitating reticulum cells within the T cell zones of lymphoid organs. Human early pregnancy decidua contains immunostimulatory CD83 antigen positive dendritic cells. Some germinal centre B cells and activated peripheral lymphocytes also express CD83 antigen. CD83 is also used as a marker for Hodgkin’s lymphoma, Reed Sternberg cells and breast carcinoma.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
CD83
Positive Control
Human brain, tonsil, lymph node or spleen. RH30 or PC-3 cell lysates.
Immunogen
Recombinant fragment corresponding to the N-terminal region of the human CD83 protein
Alternate Names
CD83 antigen, BL11|HB15

Database Links

Entrez Gene ID
9308
SwissProt
Q01151

Additional Information

Clone
r1H4b
Chromosome Location
6p23
Mol. Weight of Antigen
23.04kDa
Expression System
CHO Cells
Purification
Protein G

Related Antibodies

View All CD83 Antibodies

Functions

  • Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHCII trafficking MARCHF8. Also inhibits MARCHF1 association with MHCII or CD86 to prevent their ubiquitination and subsequent degradation (PubMed:21220452). In addition, acts as an important modulator of protective responses against acute infections (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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