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QKI / Protein Quaking Antibody [PCRP-QKI-2F10]

In Stock
Catalog Number Formulation Size Price
9444-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
9444-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
9444-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
QKI / Protein Quaking Antibody

SDS-PAGE Analysis Purified QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) Confirmation of Purity and Integrity of Antibody.

QKI / Protein Quaking Antibody - Image 2

Flow cytometric analysis of PFA-fixed HeLa cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

QKI / Protein Quaking Antibody - Image 3

Flow cytometric analysis of PFA-fixed K562 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

QKI / Protein Quaking Antibody - Image 4

Flow cytometric analysis of PFA-fixed U87 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

QKI / Protein Quaking Antibody - Image 5

Flow cytometric analysis of PFA-fixed MCF7 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

QKI / Protein Quaking Antibody - Image 6

Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

QKI / Protein Quaking Antibody - Image 7

Immunofluorescence Analysis of K562 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

QKI / Protein Quaking Antibody - Image 8

Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease, Nuclear Marker
Isotype / Light Chain
IgG2c /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
QKI
Positive Control
HeLa, K562 or MCF7 cells., U87
Immunogen
Recombinant full-length human QKI protein
Alternate Names
Protein quaking, Homolog of mouse quaking QKI KH domain RNA binding protein; HQK1; Protein quaking; QK1; QK3; QKI; QKI1; Quaking homolog KH domain RNA binding mouse; Quaking isoform 1; Quaking protein; RNA binding protein HQK

Database Links

Entrez Gene ID
9444
SwissProt
Q96PU8

Additional Information

Clone
PCRP-QKI-2F10
Chromosome Location
6q26
Mol. Weight of Antigen
37.6kDa

Related Antibodies

View All QKI Antibodies

Functions

  • RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity).

Key References

  • Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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