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Recombinant PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody [PDL1/8591R]

In Stock
Catalog Number Formulation Size Price
29126-RBM27-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
29126-RBM27-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
29126-RBM27-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody

Formalin-fixed, paraffin-embedded human placenta stained with PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/8591R). Inset: PBS instead of primary antibody; secondary only negative control.

CD274 Antibody in Western Blot (WB)

CD274 Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung tissue lysate using CD274 Rabbit Recombinant Monoclonal Antibody (PDL1/8591R).

CD274 Antibody in SDS-PAGE

CD274 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Programmed cell death 1 ligand 1 Recombinant Rabbit Monoclonal Antibody (PDL1/8591R). Confirmation of Purity and Integrity of Antibody.

CD274 Antibody in SDS-PAGE

CD274 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Programmed cell death 1 ligand 1 Recombinant Rabbit Monoclonal Antibody (PDL1/8591R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Mast Cell Marker, PD-1 blockade immunotherapy, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
CD274
Positive Control
Human placenta|spleen or tonsil tissue. Lung
Immunogen
Recombinant fragment (around aa190-290) of human CD274 protein (exact sequence is proprietary)
Alternate Names
Programmed cell death 1 ligand 1, B7 homolog 1, B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Database Links

Entrez Gene ID
29126
SwissProt
Q9NZQ7

Additional Information

Clone
PDL1/8591R
Chromosome Location
9p24.1
Mol. Weight of Antigen
37-50kDa

Related Antibodies

View All CD274 Antibodies

Functions

  • Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
  • The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Key References

  • Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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