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Recombinant MUC5AC (Mucin 5AC / Gastric Mucin) Antibody [MUC5AC/8340R]

In Stock
Catalog Number Formulation Size Price
4586-RBM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4586-RBM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4586-RBM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. Mucin 6 carries GlcNAcα1- 4Galβ-R structures, indicating that α1, 4-N acetylglucosaminyltransferase is important to the formation of the mucous glycoproteins in vivo. Mucin 5AC is a gel-forming mucin that is secreted from surface mucous cells. Glucocorticoid is required for the expression of Mucin 5AC mRNA and high doses of hydrocortisone suppresses its expression. Additionally, asthmatic fluid stimulates Mucin 5AC synthesis several-fold. The pro-inflammatory cytokines IL-6 and TNFα stimulate Mucin 5AC secretion and thus contribute to the upregulation of mucin by chronic inflammation. Expression of Mucin 5AC is retinoic acid (RA)- or retinol-dependent, and RA control of mucin genes is mediated by the retinoid acid receptor RARα and, to a lesser extent, by RARÎ �. Thyroid hormone binding to thyroid receptors inhibits Mucin 5AC gene expression. Mucin 5AC is also expressed in normal endocervical epithelium, small intestine, gastric cells (Lewis type 1) and gastric metaplasia and it is one of the major mucins in the ethmoid mucosa.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
MCF-7 cells. Human stomach.
Immunogen
Recombinant full-length human MUC5AC protein
Alternate Names
Mucin-5AC, Gastric mucin, Major airway glycoprotein, Mucin-5 subtype AC, tracheobronchial, Tracheobronchial mucin, Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MUC5AC/8340R
Chromosome Location
11p15.5
Mol. Weight of Antigen
>1|000kDa

Functions

  • Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).

Key References

  • Int. J. Cancer 47: 304-310 (1991).

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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