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Recombinant Major Vault Protein (MVP) Antibody [VP2897R]

In Stock
Catalog Number Formulation Size Price
9961-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
9961-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
9961-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Major Vault Protein (MVP) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Major Vault Protein Rabbit Recombinant Monoclonal Antibody (VP2897R).

Recombinant Major Vault Protein (MVP) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Major Vault Protein Rabbit Recombinant Monoclonal Antibody (VP2897R).

Major Vault Protein (MVP) Antibody in Western Blot (WB)

Major Vault Protein (MVP) Antibody in Western Blot (WB)

Western Blot Analysis of A549 lysate using Major Vault Protein (MVP) Rabbit Recombinant Monoclonal Antibody (VP2897R).

Major Vault Protein Antibody in Western Blot (WB)

Major Vault Protein Antibody in Western Blot (WB)

Western Blot Analysis of Human Heart, Human Liver, Human Lung and Human Spleen tissue lysates using Major Vault Protein Recombinant Rabbit Monoclonal Antibody (VP2897R).

Major Vault Protein Antibody in Western Blot (WB)

Major Vault Protein Antibody in Western Blot (WB)

Western Blot Analysis of Kidney, Spleen and Stomach tissue lysates of different species using Major Vault Protein Recombinant Rabbit Monoclonal Antibody (VP2897R).

MVP Antibody in SDS-PAGE

MVP Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Major vault protein Rabbit Monoclonal Antibody (VP2897R). Confirmation of Purity and Integrity of Antibody.

MVP Antibody in SDS-PAGE

MVP Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Major vault protein Rabbit Monoclonal Antibody (VP2897R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nuclear pore complex, Nucleus, Perinuclear region
Gene Name
MVP
Positive Control
A549, MCF-7 or HeLa cells. Breast tumors, Human Heart, Human Liver, Human Lung, Human Spleen
Immunogen
Recombinant full-length human MVP protein
Alternate Names
Major vault protein, Lung resistance-related protein, Lung resistance-related protein (LRP); Major Vault Protein; MVP; VAULT1

Database Links

Entrez Gene ID
9961
SwissProt
Q14764

Additional Information

Clone
VP2897R
Chromosome Location
16p11.2
Mol. Weight of Antigen
104-110kDa

Related Antibodies

View All MVP Antibodies

Functions

  • Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Key References

  • Abbondanza C., et al. J Cell Biol 1998;141(6):1301-1310.
  • Den Boer, M.L. et al. Blood 91, 2092-2098 (1998).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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