Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).

SDS-PAGE Analysis Purified HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4). Confirmation of Purity and Integrity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC6-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC6-1A4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Printable Datasheet

HDAC6 [PCRP-HDAC6-1A4]

Name: HDAC6
SKU: 10013-MSM1
Availability: InStock

Primary Antibodies Monoclonals Mouse Monoclonals

Summary

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.This enzyme deacetylates lysine residues in histones H2A, H2B, H3 and H4.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence

Species Reactivity

Human

Isotype

IgG2a

Cellular Localization

Axon, Cell projection, Cytoplasm, Cytoskeleton, Dendrite, Nucleus, Perikaryon

Gene Name

HDAC6

Positive Control

A549 or HeLa cells.

Immunogen

Recombinant full-length human HDAC6 protein

Alternate Names

Histone deacetylase 6, Tubulin-lysine deacetylase HDAC6, CPBHM; HDAC6; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM21; PPP1R90; Protein phosphatase 1 regulatory subunit 90

Database Links

Entrez Gene ID

10013

Unigene

6764

SwissProt

Q9UBN7

Additional Information

Clone

PCRP-HDAC6-1A4

Human Gene Symbol

HDAC6

Chromosome Location

Xp11.23

Mol. Weight of Antigen

160kDa

Functions

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385). In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).

Known Applications & Suggested Dilutions

Immunoprecipitation (1-2ug per 100-500ug of total protein)
,Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
,Optimal dilution for a specific application should be determined.

Key References

Thway K, et al. Am J Surg Pathol. 2012 Mar;36(3):462-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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