These antibodies are validated on HuProtâ„¢ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE
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Flow cytometric analysis of PFA-fixed HeLa cells. GTF2A1Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2A1-Monospecific Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml |
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Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-伪, TFIIA-尾 and TFIIA-纬, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.
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