Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Fodrin / Alpha Spectrin II (SPTAN1) / NEAS Antibody [SPTAN1/3506]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
6709-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6709-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6709-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous 伪 and 尾 chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin 伪 I and spectrin 尾 I are present in erythrocytes, whereas spectrin 伪 II (also designated fodrin 伪) and spectrin 尾 II (also designated fodrin 尾) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin 伪 II, a neuronal cytoskeleton protein.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
Positive Control
Human colon or kidney tissues (IHC).
Immunogen
Recombinant fragment of human SPTAN1 protein (around aa2351-2475) (exact sequence is proprietary))
Alternate Names
Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit, Alpha-II spectrin; brain; EIEE5; Fodrin alpha chain; NEAS; Non erythrocytic spectrin alpha; non-erythroid alpha chain; SPECA; Spectrin alpha chain brain; Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spna2; SPTA2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SPTAN1/3506
Chromosome Location
9q34.11
Mol. Weight of Antigen
240kDa

Functions

  • Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Key References

  • Wang Yet al.Critical roles of aII spectrin in brain development and epileptic encephalopathy.J Clin Invest128:760-773 (2018).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Fodrin / Alpha Spectrin II (SPTAN1) / NEAS Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK