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DCP2 (Decapping mRNA 2) Antibody [PCRP-DCP2-1D6]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
167227-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
167227-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
167227-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3'exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2a /
Cellular Localization
Cytoplasm, Nucleus, P-body
Gene Name
Positive Control
Daudi or Jurkatcells., MCF7
Immunogen
Recombinant full-length human DCP2protein
Alternate Names
m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, DCP2 decapping enzyme homolog (S. cerevisiae); DFLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-DCP2-1D6
Chromosome Location
5q22.2
Mol. Weight of Antigen
48.4kDa

Functions

  • Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12417715, PubMed:12218187, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413). Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968). The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (PubMed:28002401). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Key References

  • Wang, Z., et al. 2002. Proc. Natl. Acad. Sci. USA 99: 12663-12668.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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