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Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [rC86/6872]

In Stock
Catalog Number Formulation Size Price
942-MSM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
942-MSM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
942-MSM22-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Staining of cell surface using rC86/6872 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Staining of cell surface using rC86/6872 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (rC86/6872). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (rC86/6872). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Cytokine Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cyanelle, Plastid
Gene Name
rpoC1
Positive Control
293T or Jurkat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Immunogen
Recombinant fragment (around aa 66-195) of human CD86 protein (exact sequence is proprietary)
Alternate Names
DNA-directed RNA polymerase subunit beta', PEP, Plastid-encoded RNA polymerase subunit beta', Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2

Database Links

Entrez Gene ID
942
SwissProt
P4208

Additional Information

Clone
rC86/6872
Chromosome Location
3q13.33
Mol. Weight of Antigen
70kDa

Related Antibodies

View All rpoC1 Antibodies

Functions

  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Key References

  • Engel P et. al. Blood. 1994;84(5):1402-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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