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CD63 (Late Endosomes Marker) Antibody [MX-49.129.5]

In Stock
Best Seller
Catalog Number Formulation Size Price
967-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
967-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
967-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD63 Antibody in Immunohistochemistry (IHC (P))

CD63 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Immunohistochemistry (IHC (P))

CD63 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded mouse spleen stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Immunofluorescence (IF)

CD63 Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of HeLa cells stained using CF488-labeled-CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments labeled with phalloidin (red). Nuclei stained with DAPI (blue).

CD63 Antibody in Immunofluorescence (IF)

CD63 Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of PFA-fixed U87MG cells stained using CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of NIH/3T3 cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Immunohistochemistry (IHC (P))

CD63 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained cells (gray).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained exosomes (gray).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained cells (gray).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained exosomes (gray).

CD63 Antibody in Western Blot (WB)

CD63 Antibody in Western Blot (WB)

Western blot analysis of human spleen tissue lysate using CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow Cytometry of human CD63 on MCF-7 cells. Black: Cells alone; Grey: Isotype Control; Green: CF488-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow Cytometric staining of CD63 on human PBMC cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cell surface, Cytoplasm, Endosome, Extracellular exosome, Late endosome membrane, Lysosome membrane, Melanosome, Multivesicular body, Secreted
Gene Name
CD63
Positive Control
SK-MEL-28
Immunogen
Full length CD63 of human origin
Alternate Names
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; TSPAN30

Database Links

Entrez Gene ID
967
SwissProt
P08962

Additional Information

Clone
MX-49.129.5
Chromosome Location
12q13.2
Mol. Weight of Antigen
26kDa (core protein); 30-60kDa (glycosylated)

Related Antibodies

View All CD63 Antibodies

Functions

  • Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Key References

  • C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985.
  • AA Palmer et al., Pathology 17: 335-339, 1985.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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