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Recombinant CD52 (Epididymis-Specific Protein 5) Antibody [CD52/2276R]

In Stock
Best Seller
Catalog Number Formulation Size Price
1043-RBM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1043-RBM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1043-RBM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD52 (Epididymis-Specific Protein 5) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD52 Rabbit Recombinant Monoclonal Antibody (CD52/2276R).

Recombinant CD52 (Epididymis-Specific Protein 5) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Tonsil stained with CD52 Rabbit Recombinant Monoclonal Antibody (CD52/2276R).

CD52 Antibody in SDS-PAGE

CD52 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD55 Recombinant Rabbit Antibody (CD52/2276R).Confirmation of Purity and Integrity of Antibody.

CD52 Antibody in Western Blot (WB)

CD52 Antibody in Western Blot (WB)

Western blot analysis of Human Spleen tissue lysate using CD52 Recombinant Rabbit Monoclonal Antibody (CD52/2276R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Human CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human, Monkey
Research Areas
B Cell Markers
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CD52
Positive Control
Daudi cell lines or human tonsil., Human peripheral blood cells, Human Spleen
Immunogen
Recombinant full-length human CD52 protein
Alternate Names
CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, Cambridge pathology 1 antigen; CAMPATH 1; CAMPATH 1 antigen; CD52; CD52 antigen; CDw52; CDW52 antigen; Epididymal secretory protein E5; Epididymis secretory sperm binding protein Li 171mP; He5; Human epididymis-specific protein 5

Database Links

Entrez Gene ID
1043
SwissProt
P31358

Additional Information

Clone
CD52/2276R
Chromosome Location
1p36.11
Mol. Weight of Antigen
6kDa

Related Antibodies

View All CD52 Antibodies

Functions

  • May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

Key References

  • Hale G et al. Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. Blood. 1983 Oct;62(4):873-82.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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