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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Antibody [C209/6774]

In Stock
Catalog Number Formulation Size Price
30835-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
30835-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
30835-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells

Summary

DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
Positive Control
THP-1 or HeLa cells (IF/FACS).
Immunogen
Recombinant human CD209 protein fragment (exact sequence is proprietary)
Alternate Names
CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, CD209; CDSIGN; CIRE; CLEC4L; DC-SIGN; DC-SIGN1; DCSIGN; Dendritic cell-specific ICAM-3 Grabbing Non-integrin 1; HIV GP120 Binding Protein; SIGN-R1; SIGNR5

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
C209/6774
Chromosome Location
19p13
Mol. Weight of Antigen
45kDa

Functions

  • Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.
  • On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2.
  • (Microbial infection) Acts as an attachment receptor for Ebolavirus.
  • (Microbial infection) Acts as an attachment receptor for Cytomegalovirus.
  • (Microbial infection) Acts as an attachment receptor for HCV.
  • (Microbial infection) Acts as an attachment receptor for Dengue virus.
  • (Microbial infection) Acts as an attachment receptor for Measles virus.
  • (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1.
  • (Microbial infection) Acts as an attachment receptor for Influenzavirus A.
  • (Microbial infection) Acts as an attachment receptor for SARS-CoV.
  • (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.
  • (Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed:23966408). Acts as an attachment receptor for Marburg virusn.
  • (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus.
  • (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus.
  • (Microbial infection) Acts as an attachment receptor for West-nile virus.
  • (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed:16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed:16092920, PubMed:21203928).

Key References

  • Steinman, R.M. 2000. DC-SIGN: A guide to some mysteries of Dendritic cells. Cell 100: 491-494.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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