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Recombinant CD10 (Membrane Metalloendopeptidase) Antibody [MME/8376R]

In Stock
Catalog Number Formulation Size Price
4311-RBM38-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
4311-RBM38-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
4311-RBM38-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD10 (Membrane Metalloendopeptidase) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with CD10 Recombinant Rabbit Monoclonal Antibody (MME/8376R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant CD10 (Membrane Metalloendopeptidase) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MME/8376R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

CD10 Antibody in Western Blot (WB)

CD10 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney lysate using CD10 Rabbit Recombinant Monoclonal Antibody (MME/8376R).

CD10Antibody in SDS-PAGE

CD10Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD10 Recombinant Rabbit Monoclonal Antibody (MME/8376R ). Confirmation of Purity and Integrity of Antibody.

CD10 Antibody in Western Blot (WB)

CD10 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine and Rabbit Intestine tissue lysates using CD10 Rabbit Recombinant Monoclonal Antibody (MME/8376R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
Research Areas
Cardiovascular, Immunology, B Cell Markers, Hematopoietic Stem Cells
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
MME
Positive Control
Human small intestine or Human kidney.|Human Tonsil
Immunogen
Recombinant human CD10 protein fragment (aa 1-500) (exact sequence is proprietary)
Alternate Names
Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, Atriopeptidase; Common acute lymphocytic leukemia antigen (CALLA); Enkephalinase (EPN); gp100; Membrane metalloendopeptidase (MME); Neprilysin; Neutral endopeptidase (NEP); Skin fibroblast elastase (SFE)

Database Links

Entrez Gene ID
4311
SwissProt
P08473

Additional Information

Clone
MME/8376R
Chromosome Location
3q25.2
Mol. Weight of Antigen
100kDa

Related Antibodies

View All MME Antibodies

Functions

  • Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:6349683, PubMed:6208535, PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:6349683, PubMed:17101991). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:6349683, PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:2531377, PubMed:2972276, PubMed:16254193). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

Key References

  • SuzukoM., et al. Mod Pathol 2002;15(4):397-405.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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