Recombinant Adipophilin / Perilipin-2 (Marker of Lipid Accumulation) [rADFP/1493]

Clear

Summary

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Product Properties & Targets

Host
Applications
Species Reactivity
Isotype
Cellular Localization
Lipid droplet, Membrane
Light Chain
Gene Name
Positive Control
adrenal gland or cerebellum (IHC)., Liver
Immunogen
Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)
Alternate Names
Perilipin-2, Adipophilin, Adipose differentiation-related protein, Adipophilin; ADFP; Adipose differentiation-related protein (ADRP); Perilipin-2 (PLIN2)

Database Links

Entrez Gene ID
123
Unigene
SwissProt

Additional Information

Clone
rADFP/1493
Human Gene Symbol
PLIN2
Chromosome Location
9p22.1
Mol. Weight of Antigen
48kDa

Functions

  • Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Kern, P.A., et al. 2004. Perilipin expression in human adipose tissue is elevated with obesity. J. Clin. Endocrinol. Metab. 89: 1352-1358.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Adipophilin / Perilipin-2 (Marker of Lipid Accumulation)”

Your email address will not be published. Required fields are marked *

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies