|Catalog No||Formulation||Size||Price (USD)|
|7535-MSM6-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|7535-MSM6-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|7535-MSM6-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||7535|
|Human Gene Symbol||ZAP70|
|Human Chromosome Location||2q12|
|Synonyms||Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70;TZK; Zeta chain associated protein kinase 70kDa|
|Immunogen||Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2a, kappa|
|Mol. Weight of Antigen||70kDa|
|Cellular Localization||Cell Surface and Cytoplasmic|
|Species Reactivity||Human. Others-not known|
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Jurkat cells.Tonsil or lymph node.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.