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Home >> Antibodies >> ZAP70(Chronic Lymphocytic Leukemia Marker)

ZAP70 (Chronic Lymphocytic Leukemia Marker)

Mouse Monoclonal Antibody [Clone ZAP70/2046]

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Catalog No Formulation Size Price (USD)  
7535-MSM6-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
7535-MSM6-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
7535-MSM6-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 7535
Human SwissProt P43403
Human Unigene 234569
Human Gene Symbol ZAP70
Human Chromosome Location 2q12
Synonyms Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70;TZK; Zeta chain associated protein kinase 70kDa
Immunogen Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2a, kappa
Mol. Weight of Antigen 70kDa
Cellular Localization Cell Surface and Cytoplasmic
Species Reactivity Human. Others-not known

Specificity & Comments

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Jurkat cells.Tonsil or lymph node.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Iwashima M, Irving BA, van Oers NSC, Chan AC, Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 1994;263:1136-9.
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