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Home >> Antibodies >> Vinculin (Marker of Age-related Macular Degeneration)

Vinculin (Marker of Age-related Macular Degeneration)

Mouse Monoclonal Antibody [Clone VCL/2573]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Mouse Monoclonal Antibody (VCL/2573).

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SDS-PAGE Analysis Purified Vinculin Mouse Monoclonal Antibody (VCL/2573). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Vinculin Monoclonal Antibody (VCL/2573)

Product Details

Synonyms

CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; VCL; VINC

Positive Control

Human Bladder and Testis.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

117kDa

Specificity & Comments

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin each contain Actin binding sites. Reportedly, vinculin is a potential plasma biomarker for Age-related Macular Degeneration (AMD). The early detection of AMD using novel plasma biomarkers with genetic modeling may enable timely treatment and vision preservation in the elderly.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kim H-J, et al. Identication of vinculin as a potential plasma marker for age-related macular degeneration. Invest Ophthalmol Vis Sci. 2014;55:7166-7176.

Bioinformatics

  •  Entrez Gene ID: 7414
  •  Gene Symbol: VCL
  •  SwissProt: P18206
  •  Chromosome Location: 10q22.2
  •  Unigene: 643896
NBT02573