Formalin-fixed, paraffin-embedded human uterine carcinoma stained with Vimentin Mouse Monoclonal Antibody (V9).
SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (V9). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of U-87 cell lysate using Vimentin Mouse Monoclonal Antibody (V9).
Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Mouse Monoclonal Antibody (V9).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.