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Home >> Antibodies >> Vimentin (Mesenchymal Cell Marker)

Vimentin (Mesenchymal Cell Marker)

Mouse Monoclonal Antibody [Clone V9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human uterine carcinoma stained with Vimentin Mouse Monoclonal Antibody (V9).

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SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (V9). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of U-87 cell lysate using Vimentin Mouse Monoclonal Antibody (V9).

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Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Mouse Monoclonal Antibody (V9).

Product Details

Synonyms

VIM

Positive Control

U-87, Raji, Jurkat or HeLa cells. Lymph node or tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Porcine Lens

Cellular Localization

Cytoplasmic

Species Reactivity

Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

57-60kDa

Specificity & Comments

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

Bioinformatics

  •  Entrez Gene ID: 7431
  •  Gene Symbol: VIM
  •  SwissProt: P08670
  •  Chromosome Location: 10p12.33
  •  Unigene: 455493
NBT02775