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Home >> Antibodies >> Prostate Specific Antigen (PSA)

Prostate Specific Antigen (PSA)

Recombinant Rabbit MonoclonalAntibody [Clone KLK3/4602R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA Recombinant Rabbit MonoclonalAntibody (KLK3/4602R).

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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA Recombinant Rabbit Monoclonal Antibody (KLK3/4602R).

img

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KLK3/4602R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Positive Control

PC12 cells.Normal prostate or prostate carcinoma tissues.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Synthetic peptide corresponding to PSA residues within aa150-250 of PSA was used as an immunogen.

Cellular Localization

Secreted

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

29kDa

Specificity & Comments

Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999).

Bioinformatics

  •  Entrez Gene ID: 354
  •  Gene Symbol: KLK3
  •  SwissProt: P07288
  •  Chromosome Location: 19q13.33
  •  Unigene: 171995