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Home >> Antibodies >> p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)

Rabbit Polyclonal Antibody

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p40 Rabbit Polyclonal Antibody.

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Formalin-fixed, paraffin-embedded Lung Squamous Cell Carcinoma stained with p40 Rabbit Polyclonal Antibody.

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Western Blot of HEK293 lysate (1) Absence and (2) Presence of immunizing peptide p40 Rabbit PAb.

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SDS-PAGE Analysis Purified p40 Rabbit Polyclonal Antibody.

Product Details

Synonyms

deltaNp63, TP63

Positive Control

HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat. Cow.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

40kDa

Specificity & Comments

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Related Products



200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Bishop, JA et. al. Modern Pathology 25 : 405-4152. Scagliotti G et. al. J Thorac Oncol 6:64-70. 3. Kargi A et. al. Appl Immunohistochem Mol Morphol 15:415-420.

Bioinformatics

  •  Entrez Gene ID: 8626
  •  Gene Symbol: TP63
  •  SwissProt: Q9H3D4
  •  Chromosome Location: 3q28
  •  Unigene: 137569
NBT00375