Formalin fixed paraffin embedded human colon carcinoma stained with p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCIP1/6907 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).