Home >> Antibodies >> p21WAF1 (Tumor Suppressor Protein)

p21WAF1 (Tumor Suppressor Protein)

Recombinant Mouse Monoclonal Antibody [Clone rCIP1/6907]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin fixed paraffin embedded human colon carcinoma stained with p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCIP1/6907 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907). Confirmation of Purity and Integrity of Antibody.

Formalin fixed paraffin embedded human colon carcinoma stained with p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCIP1/6907 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified p21 Recombinant Mouse Monoclonal Antibody (rCIP1/6907). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, PIC1, SDI1, SLC12A9, p21Cip1/Waf1, Wild type p53 activated fragment 1 (WAF1)

Positive Control

HeLa cells. Human skin, colon, or breast carcinoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-100) of human p21 (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

21kDa

Specificity & Comments

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).