2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> NFIA / NF1A (Nuclear Factor 1A) (Transcription Factor)

NFIA / NF1A (Nuclear Factor 1A) (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-NFIA-2C6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow cytometric analysis of PFA-fixed HeLa cells. NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

img

SDS-PAGE Analysis Purified NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). Confirmation of Integrity and Purity of Antibody.

img

Formalin-fixed, paraffin-embedded human lung stained with NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6).

Product Details

Synonyms

CCAAT-box-binding transcription factor; CTF; NF I/A; NF-I/A; NF1-A; NFI A; NFI L; NFIA; Nuclear factor 1 A type; Nuclear factor 1 A-type; TGGCA binding protein

Positive Control

HeLa cells. Human fetal gut, colon, liver or heart.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF, Western Blot, IHC-P         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human NFIA protein corresponding to protein domain

Cellular Localization

Nucleus.

Species Reactivity

Human, Mouse and Rat.

Host / Ig Isotype

Mouse / IgG2c, kzppz

Mol. Weight of Antigen

55kDa

Specificity & Comments

NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, -globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.

Bioinformatics

  •  Entrez Gene ID: 4774
  •  Gene Symbol: NFIA
  •  SwissProt: Q15784
  •  Chromosome Location: 1p31.3
  •  Unigene: 322431