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Home >> Antibodies >> NEUROG3 / Neurogenin 3 / NGN3(Transcription Factor)

NEUROG3 / Neurogenin 3 / NGN3(Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-NEUROG3-1E10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. NEUROG3 Mouse Monoclonal Antibody (PCRP-NEUROG3-1E10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NEUROG3 Mouse Monoclonal Antibody (PCRP-NEUROG3-1E10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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SDS-PAGE Analysis NEUROG3 Mouse Monoclonal Antibody (PCRP-NEUROG3-1E10). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using NEUROG3-Monospecific Mouse Monoclonal Antibody (PCRP-NEUROG3-1E10).

Product Details

Synonyms

ATH4B; Atoh5; bHLHa7; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; Neurogenin-3; Neurogenin3; NGN-3; ngn3; Protein atonal homolog 5

Positive Control

HeLa cells. Human fetal gut, colon, liver or heart.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-lengthNEUROG3protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

23.54kDa

Specificity & Comments

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Jacquemin, P., et al. 2000. Transcription factor hepatocyte nuclear factor 6regulates pancreatic endocrine cell differentiation and controls expressionof the proendocrine gene ngn3. Mol. Cell. Biol. 20: 4445-4454.

Bioinformatics

  •  Entrez Gene ID: 50674
  •  Gene Symbol: NEUROG3
  •  SwissProt: Q9Y4Z2
  •  Chromosome Location: 10q22.1
  •  Unigene: 288655