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NEUROG3 / Neurogenin 3 / NGN3 (Transcription Factor) Antibody [PCRP-NEUROG3-1E10]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
50674-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
50674-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
50674-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells

Summary

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus
Gene Name
Positive Control
Colon, HeLa cells. Human fetal gut, liver or heart.
Immunogen
Recombinant human full-lengthNEUROG3protein
Alternate Names
Neurogenin-3, Class A basic helix-loop-helix protein 7, Protein atonal homolog 5, ATH4B; Atoh5; bHLHa7; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; Neurogenin-3; Neurogenin3; NGN-3; ngn3; Protein atonal homolog 5

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-NEUROG3-1E10
Chromosome Location
10q22.1
Mol. Weight of Antigen
23.54kDa

Functions

  • Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity).

Key References

  • Jacquemin, P., et al. 2000. Transcription factor hepatocyte nuclear factor 6regulates pancreatic endocrine cell differentiation and controls expressionof the proendocrine gene ngn3. Mol. Cell. Biol. 20: 4445-4454.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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