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Home >> Antibodies >> Myogenin / Myf-4 (Skeletal Muscle Marker)

Myogenin / Myf-4 (Skeletal Muscle Marker)

Mouse Monoclonal Antibody [Clone MGN185]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Mouse Monoclonal Antibody (MGN185).

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SDS-PAGE Analysis Purified Myogenin Mouse Monoclonal Antibody (MGN185). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Positive Control

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human myogenin recombinant protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat. Cat. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.

Bioinformatics

  •  Entrez Gene ID: 4656
  •  Gene Symbol: MYOG
  •  SwissProt: P15173
  •  Chromosome Location: 1q32.1
  •  Unigene: 2830
NBT00185