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Recombinant Myogenin / Myf-4 (Skeletal Muscle Marker) Antibody [rMYOG/6297]

Additional information

Catalog Number: 4656-MSM7
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4656-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4656-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4656-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Myogenin / Myf-4 (Skeletal Muscle Marker) Antibody

Formalin-fixed, paraffin-embedded human Rhabdomyosarcomastained with Myogenin Recombinant Mouse Monoclonal Antibody (rMYOG/6297). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Myogenin / Myf-4 (Skeletal Muscle Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified MyogeninRecombinant Mouse Monoclonal Antibody (rMYOG/6297). Confirmation of Integrity and Purity of Antibody.

MYOG Antibody in Western Blot (WB)

MYOG Antibody in Western Blot (WB)

Western Blot Analysis of Human Skeletal muscle tissue lysate using MYOG Recombinant Mouse Monoclonal Antibody (rMYOG/6297).

MYOG Antibody in Western Blot (WB)

MYOG Antibody in Western Blot (WB)

Western Blot Analysis of Skeletal muscle tissue lysates of different species using MYOG Recombinant Mouse Monoclonal Antibody (rMYOG/6297).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rMYOG/6297
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
MYOG
Positive Control
Rh-30 or HeLa cells. Skeletal muscle or rhabdomyosarcoma. Skeletal muscle.
Immunogen
Recombinant full-length human myogenin (MYOG) protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in the CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
Key References
  • Wang NP et. al. Am J Pathol 1995, 147:1799-1810.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4656
SwissProt
P15173
Chromosome Location
1q32.1
Mol. Weight of Antigen
34kDa
Alternate Names
Myogenin, Class C basic helix-loop-helix protein 3, Myogenic factor 4, bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Summary

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

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