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Recombinant MMP9 (Matrix Metalloproteinase 9) Antibody [MMP9/2025R]

In Stock
Catalog Number Formulation Size Price
4318-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4318-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4318-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human spleen stained with MMP9 Rabbit Recombinant Monoclonal Antibody (MMP9/2025R).

Formalin-fixed, paraffin-embedded human spleen stained with MMP9 Rabbit Recombinant Monoclonal Antibody (MMP9/2025R).

SDS-PAGE Analysis of Purified MMP9 Rabbit Recombinant Monoclonal Antibody (MMP9/2025R).

SDS-PAGE Analysis of Purified MMP9 Rabbit Recombinant Monoclonal Antibody (MMP9/2025R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is down-regulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Cardiovascular, Developmental Biology, Immunology, Angiogenesis, BBB VCAM-1 Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
MMP9
Positive Control
U937 cells. Spleen or heart.
Immunogen
Recombinant fragment (around aa 603-614) of human MMP-9 protein (exact sequence is proprietary)
Alternate Names
Matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; CLG 4B; CLG4B; Collagenase Type 4 beta; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase B; Gelatinase beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; MMP 9; MMP9; Type V collagenase

Database Links

Entrez Gene ID
4318
SwissProt
P14780

Additional Information

Clone
MMP9/2025R
Chromosome Location
20q13.12
Mol. Weight of Antigen
92kDa

Related Antibodies

View All MMP9 Antibodies

Functions

  • Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-
  • -Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.

Key References

  • Thanakit V, Sampatanukul P, Ruangvejvorachai P, Keelawat S. The association of co-expression of CD44v4/MMP-9 with different nodal status in high-grade breast carcinoma patients. J Med Assoc Thai. 2005 Sep;88 Suppl 4:S30-5.
  • Elsasser, A., et al. 2004. Human hibernating myocardium is jeopardized by apoptotic and autophagic cell death. J. Am. Coll. Cardiol. 43: 2191-2199.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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