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Home >> Antibodies >> MEF2B(Transcriptional Activator)

MEF2B(Transcriptional Activator)

Mouse Monoclonal Antibody [Clone PCRP-MEF2B-2F9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Immunofluorescence analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MEF2B-Monospecific Mouse Monoclonal Antibody (PCRP-MEF2B-2F9).

Product Details

Synonyms

MADS box transcription enhancer factor 2; MDS1 EVI1; Mef2b; Myocyte enhancer factor 2B; Myocyte-specific enhancer factor 2B; PRDM3; RSRFR2; Serum response factor like protein 2; XMEF2

Positive Control

HeLa or Ramos cells.

Known Applications & Suggested Dilutions

IP, Western Blot, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MEF2B protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

160kDa

Specificity & Comments

The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members, termed MEF-2A, -2B, -2C, and -2D, arise from alternatively spliced transcripts. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. The MEF-2 family represents a second class of DNA binding regulatory proteins. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hidaka, K., et al. 1995. The MEF2B homologue differentially expressed inmouse embryonal carcinoma cells. Biochem. Biophys. Res. Commun. 213:555-560.

Bioinformatics