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JAZF1 (Transcription Corepressor) Antibody [PCRP-JAZF1-1C2]

In Stock
Catalog Number Formulation Size Price
221895-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
221895-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
221895-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2a /
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or U87 cells.Ubiquitous tissue expression.
Immunogen
Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)
Alternate Names
Juxtaposed with another zinc finger protein 1, TAK1-interacting protein 27, Zinc finger protein 802, DKFZp761K2222, TIP27, ZNF802

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-JAZF1-1C2
Chromosome Location
7p15.2-p15.1
Mol. Weight of Antigen
27.08kDa

Functions

  • Acts as a transcriptional corepressor of orphan nuclear receptor NR2C2 (PubMed:15302918). Inhibits expression of the gluconeogenesis enzyme PCK2 through inhibition of NR2C2 activity (By similarity). Also involved in transcriptional activation of NAMPT by promoting expression of PPARA and PPARD (By similarity). Plays a role in lipid metabolism by suppressing lipogenesis, increasing lipolysis and decreasing lipid accumulation in adipose tissue (By similarity). Plays a role in glucose homeostasis by improving glucose metabolism and insulin sensitivity (By similarity).

Key References

  • Koontz, J.I., et al. 2001. Frequent fusion of the JAZF1 and JJAZ1 genes inendometrial stromal tumors. Proc. Natl. Acad. Sci. USA 98: 6348-6353.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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