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Home >> Antibodies >> JAZF1(Transcription Corepressor)

JAZF1(Transcription Corepressor)

MouseMonoclonal Antibody [Clone PCRP-JAZF1-1C2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence analysis of PFA-fixed HeLa cells. JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Flow cytometric analysis of PFA-fixed HeLa cells. JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Formalin-fixed, paraffin-embedded human testis stained. Strong nuclear staining using JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2).

Product Details

Synonyms

DKFZp761K2222, TIP27, ZNF802

Positive Control

HeLa or U87 cells.Ubiquitous tissue expression.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF, Western Blot, IHC-P         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to react in Mouse.

Host / Ig Isotype

Mouse/ IgG2a

Mol. Weight of Antigen

27.08kDa

Specificity & Comments

JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Koontz, J.I., et al. 2001. Frequent fusion of the JAZF1 and JJAZ1 genes inendometrial stromal tumors. Proc. Natl. Acad. Sci. USA 98: 6348-6353.

Bioinformatics

  •  Entrez Gene ID: 221895
  •  Gene Symbol: JAZF1
  •  SwissProt: Q86VZ6
  •  Chromosome Location: 7p15.2-p15.1
  •  Unigene: 368944