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Home >> Antibodies >> HLA-A (MHC I)

HLA-A (MHC I)

Mouse Monoclonal Antibody [Clone 108-2C5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified HLA-A Mouse Monoclonal Antibody (108-2C5). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of Raji cells. HLA-A Mouse Monoclonal Antibody (108-2C5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Synonyms

HLA-A; HLA class 1A; MHC class I HLA-A

Positive Control

Raji, THP-1 and Jurkat cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Normal human peripheral blood lymphocytes

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~41kDa

Specificity & Comments

HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the 1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Young NT et al. Hum Immunol 1997, 52(1):1-
  2. Domenech N et al. Hum Immunol 1991, 30(2):140-6
  3. Lozano F et al. Immunogenetics 1989, 30::50-3
  4. Lozano F et al. Tissue Antigens 1990, 35:193-5

Bioinformatics

NBT01095