2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> HDAC6

HDAC6

Mouse Monoclonal Antibody [Clone PCRP-HDAC6-1A4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

img

Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).

img

SDS-PAGE Analysis Purified HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4). Confirmation of Purity and Integrity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC6-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC6-1A4).

Product Details

Synonyms

CPBHM; HDAC6; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM21; PPP1R90; Protein phosphatase 1 regulatory subunit 90

Positive Control

A549 or HeLa cells.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HDAC6 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

160kDa

Specificity & Comments

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.This enzyme deacetylates lysine residues in histones H2A, H2B, H3 and H4.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Thway K, et al. Am J Surg Pathol. 2012 Mar;36(3):462-9.

Bioinformatics

  •  Entrez Gene ID: 10013
  •  Gene Symbol: HDAC6
  •  SwissProt: Q9UBN7
  •  Chromosome Location: Xp11.23
  •  Unigene: 6764