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Home >> Antibodies >> GTF2H2C (Transcription Factor)

GTF2H2C (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-GTF2H2C-2C9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

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SDS-PAGE AnalysisPurified GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

EVR6; PFM14; PR domain zinc finger protein 17 (PRDM17); RP72; Zinc finger protein 408 (GTF2H2C)

Positive Control

HeLa or Raji cells. Human breast or prostate carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GTF2H2C protein

Cellular Localization

Nucleus. Nuclear speckles.

Species Reactivity

Human. Predicted to react in Mouse and Rat.

Host / Ig Isotype

Mouse / IgG2c

Mol. Weight of Antigen

44kDa

Specificity & Comments

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gabriel CH, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291(46):24172-24187.

Bioinformatics

  •  Entrez Gene ID: 730394
  •  Gene Symbol: GTF2H2C
  •  SwissProt: Q6P1K8
  •  Chromosome Location: 5q13.2
  •  Unigene: 422901