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Home >> Antibodies >> GTF2H2 / BTF2 / TFIIH Basal Transcription Factor

GTF2H2 / BTF2 / TFIIH Basal Transcription Factor

Mouse Monoclonal Antibody [Clone PCRP-GTF2H2-1B9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain (red).

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Immunofluorescence Analysis of PFA-fixed MCF7 cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain (red).

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SDS-PAGE Analysis Purified GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9).

Product Details

Synonyms

Basic transcription factor 2 44 kDa subunit; BTF2; BTF2-p44; BTF2P44; General transcription factor IIH, polypeptide 2, 44kDa; T-BTF2P44; TFIIH basal transcription factor complex p44 subunit

Positive Control

HeLa or MCF7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GTF2H2 protein

Cellular Localization

Nucleus

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

44kDa

Specificity & Comments

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and Mat1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Conaway, R.C., et al. 1989. Proc. Natl. Acad. Sci. USA 86: 7356-7360.
  2. Weeda, G., et al. 1990. Cell 62: 777-791.

Bioinformatics

  •  Entrez Gene ID: 2966
  •  Gene Symbol: GTF2H2
  •  SwissProt: Q13888
  •  Chromosome Location: 5q13.2
  •  Unigene: 607501