Flow cytometric analysis of PFA-fixed HeLa cells. GTF2A1Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
SDS-PAGE Analysis Purified GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2A1-Monospecific Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, TFIIA- and TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.