2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> GTF2A1/ TFIIA (Transcription Factor)

GTF2A1/ TFIIA (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-GTF2A1-1F2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow cytometric analysis of PFA-fixed HeLa cells. GTF2A1Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

img

SDS-PAGE Analysis Purified GTF2A1 Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2). Confirmation of Purity and Integrity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2A1-Monospecific Mouse Monoclonal Antibody (PCRP-GTF2A1-1F2).

Product Details

Synonyms

General transcription factor IIA 1 19/37kDa; General transcription factor IIA subunit 1; Glucose regulated protein 58kD pseudogene; TFIIA alpha p55, isoform 1; TFIIA p19 subunit; TFIIA p35 subunit; TFIIAL; Transcription initiation factor IIA beta chain; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit

Positive Control

HeLa or MCF-7 cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GTF2A1 protein

Cellular Localization

Nucleus

Species Reactivity

Human. Predicted to react in Mouse and Rat.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

41.51kDa

Specificity & Comments

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, TFIIA- and TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 2957
  •  Gene Symbol: GTF2A1
  •  SwissProt: P52655
  •  Chromosome Location: 14q31.1
  •  Unigene: 592334