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Home >> Antibodies >> DAXX (Transcriptional Corepressor)

DAXX (Transcriptional Corepressor)

Mouse Monoclonal Antibody [Clone PCRP-DAXX-6A8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified DAXX Mouse Monoclonal Antibody (PCRP-DAXX-6A8). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence analysis of PFA-fixed HeLa cells. DAXX Mouse Monoclonal Antibody (PCRP-DAXX-6A8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Flow cytometric analysis of PFA-fixed HeLa cells. DAXX Mouse Monoclonal Antibody (PCRP-DAXX-6A8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using DAXX Mouse Monoclonal Antibody (PCRP-DAXX-6A8).

Product Details

Synonyms

DAP6; EAP1; BING2; SMIM40

Positive Control

HeLa or U87 cells. Ubiquitous tissue expression.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human DAXX protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2c

Mol. Weight of Antigen

81.37kDa

Specificity & Comments

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhong, S., et al. 2000. Role of SUMO-1-modified PML in nuclear body formation. Blood 95: 2748-2752.

Bioinformatics

  •  Entrez Gene ID: 1616
  •  Gene Symbol: DAXX
  •  SwissProt: Q9UER7
  •  Chromosome Location: 6p21.32
  •  Unigene: 336916