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Home >> Antibodies >> CHEK2

CHEK2

Mouse Monoclonal Antibody [Clone PCRP-CHEK2-1A4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence analysis of PFA-fixed HeLa cells. CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4) followed by goat anti-mouse IgG-CF488 (green). Microtubules stained with phalloidin.

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Immunofluorescence analysis of PFA-fixed K562 cells. CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4) followed by goat anti-mouse IgG-CF488 (green). Phalloidin (red).

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Flow cytometric analysis of PFA-fixed HeLa cells. CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4).

Product Details

Synonyms

Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; CHK2 checkpoint homolog (S. pombe); hCds1; HuCds 1; LFS2; PP1425; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2

Positive Control

HeLa or HCT 116cells.Human testis, spleen or colon.

Known Applications & Suggested Dilutions

IP, Flow Cyt, Western Blot, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CHEK2 corresponding to protein domain

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to react with Mouse and Rat.

Host / Ig Isotype

Mouse/ IgG2b

Mol. Weight of Antigen

60.91kDa

Specificity & Comments

Recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sutcliffe EG, et al. Cancer Genet, 2020 Aug. PMID 32805687

Bioinformatics

  •  Entrez Gene ID: 11200
  •  Gene Symbol: CHEK2
  •  SwissProt: O96017
  •  Chromosome Location: 22q12.1
  •  Unigene: 291363, 505297