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Home >> Antibodies >> CD79a (B-Cell Marker)

CD79a (B-Cell Marker)

Mouse Monoclonal Antibody [Clone JCB117]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD79a Mouse Monoclonal Antibody (JCB117).

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SDS-PAGE Analysis Purified CD79a Mouse Monoclonal Antibody (JCB117). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human Raji cell lysate using CD79a Mouse Monoclonal Antibody (JCB117).

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Flow Cytometric Analysis of Raji cells. CD79a Mouse Monoclonal Antibody (JCB117) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of paraformaldehyde-fixed Raji cells. CD79a Mouse Monoclonal Antibody (JCB117) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Product Details

Synonyms

B lymphocyte-specific MB1 protein, B-cell antigen receptor complex-associated protein alpha chain, CD79a molecule immunoglobulin associated alpha, Ig-alpha, IGA, IgM-alpha, Immunoglobulin-associated alpha, Ly54, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Positive Control

Raji, Daudi or Ramos cells. Germinal center B- cells in a lymph node or tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Western Blot 1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

44kDa

Specificity & Comments

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mason, DY, et al. 1995. Blood 86: 1453-1459.

Bioinformatics

  •  Entrez Gene ID: 973
  •  Gene Symbol: CD79A
  •  SwissProt: P11912
  •  Chromosome Location: 19q13.2
  •  Unigene: 631567
NBT00476