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Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Mouse Monoclonal Antibody [Clone RIV9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Flow Cytometric Analysis of Jurkat cells using CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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SDS-PAGE Analysis CD3e Mouse Monoclonal Antibody (RIV9). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Positive Control

Jurkat cells. Tonsil and lymph node.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human peripheral lymphocytes

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG3, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.

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200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 199
  2. Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200
  3. Vaessen LM et al. RIV-9: a Mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7
  4. Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal Mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46
  5. Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989

Bioinformatics

  •  Entrez Gene ID: 916
  •  Gene Symbol: CD3E
  •  SwissProt: P07766
  •  Chromosome Location: 11q23.3
  •  Unigene: 3003
NBT00322