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Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Mouse Monoclonal Antibody [Clone C3e/2478]

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Catalog No Formulation Size Price (USD)  
916-MSM14-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
916-MSM14-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
916-MSM14-P1ABX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 916
Human SwissProt P07766
Human Unigene 3003
Human Gene Symbol CD3E
Human Chromosome Location 11q23.3
Synonyms CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
Immunogen Recombinant human CD3e fragment (around aa 23-119) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 20kDa
Cellular Localization Cell Surface and Cytoplasmic
Species Reactivity Human. Chimpanzee.

Specificity & Comments

Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Beverley PC, Callard RE. Eur J Immunol. 1981; 11(4):329-334.
  2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.
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