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Home >> Antibodies >> CD163 (Monocyte & Macrophage Marker)

CD163 (Monocyte & Macrophage Marker)

Mouse Monoclonal Antibody [Clone M130/2164]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lymph Node stained with CD163 Mouse Monoclonal Antibody (M130/2164).

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Formalin-fixed, paraffin-embedded human Lymph Node in Colon stained with CD163 Mouse Monoclonal Antibody (M130/2164).

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SDS-PAGE Analysis Purified CD163 Mouse Monoclonal Antibody (M130/2164). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD163 Mouse Monoclonal Antibody (M130/2164).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD163 Mouse Monoclonal Antibody (M130/2164).

Product Details

Synonyms

CD163; CD163 antigen, Macrophage-associated antigen; M130, CD163 molecule; Hemoglobin scavenger receptor, MM130; Scavenger receptor cysteine rich type 1 protein M130

Positive Control

HPBL cells. Skin, Lung, Placenta or Histiocytoma.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

140kDa

Specificity & Comments

This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sean K. Lau, et al. Am J Clin Pathol 2004;122:794-801.

Bioinformatics

  •  Entrez Gene ID: 9332
  •  Gene Symbol: CD163
  •  SwissProt: Q86V7
  •  Chromosome Location: 12p13.3
  •  Unigene: 504641
NBT02164