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Home >> Antibodies >> CD163 (Monocyte & Macrophage Marker)

CD163 (Monocyte & Macrophage Marker)

Mouse Monoclonal Antibody [Clone M130/2164]

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Catalog No Formulation Size Price (USD)  
9332-MSM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
9332-MSM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
9332-MSM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 9332
Human SwissProt Q86VB7
Human Unigene 504641
Human Gene Symbol CD163
Human Chromosome Location 12p13.3
Synonyms CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 molecule, Hemoglobin scavenger receptor, MM130; Scavenger receptor cysteine rich type 1 protein M130
Immunogen Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 140kDa
Cellular Localization Cell Surface & Cytoplasmic
Species Reactivity Human. Others not known.

Specificity & Comments

This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

hPBL Cells. Skin, Lung, Placenta or Histiocytoma.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Sean K. Lau, et al. Am J Clin Pathol 2004;122:794-801.